Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by dithiothreitol | Giardia intestinalis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 92% inhibition at 0.1 mg/ml | Giardia intestinalis | |
Hg2+ | complete inhibition at 0.1 mg/ml | Giardia intestinalis | |
p-mercuribenzoate | 99% inhibition at 0.1 mg/ml | Giardia intestinalis | |
Quinacrine | 51% inhibition at 0.1 mg/ml | Giardia intestinalis | |
Zn2+ | 89% inhibition at 0.1 mg/ml | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADH | at pH 7.8 and 25°C | Giardia intestinalis | |
0.0874 | - |
NADPH | at pH 7.8 and 25°C | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
214000 | - |
gel filtration | Giardia intestinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | Giardia intestinalis | - |
NAD+ + H2O | - |
? | |
NADPH + H+ + O2 | Giardia intestinalis | - |
NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-Sepharose column chromatography | Giardia intestinalis |
Storage Stability | Organism |
---|---|
-70°C, 50% (v/v) glycerol, at least 2 months, no loss of activity | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | - |
Giardia intestinalis | NAD+ + H2O | - |
? | |
NADPH + H+ + O2 | - |
Giardia intestinalis | NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Giardia intestinalis |
homotetramer | 4 * 50300, calculated from amino acid sequence | Giardia intestinalis |
Synonyms | Comment | Organism |
---|---|---|
NOX | - |
Giardia intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Giardia intestinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 0.91 mol FAD per mol of monomer | Giardia intestinalis |